Coding

Part:BBa_K2719011

Designed by: Juan Carlos Rueda Silva & Arantxa Karam Coppola   Group: iGEM18_TecCEM   (2018-10-10)


Collagen coding device with blue chromoprotein

This part facilitates the selection of transformed colonies for the production of collagen (BBa_K2719007) by the incorporation of a reporter cassette (BBa_K592015), which constitutively expresses blue chrome protein. This reporter simplifies the selection of colonies by the appearance of blue colored colonies, serving as transformation and ligation control. This part has both reporter genes, RFP (BBa_I13521) and Blue chromoprotein, so it could be easier to detect and select colonies of our interest.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 848
    Illegal NheI site found at 871
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1887


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Categories
Parameters
None